羊抗小鼠IgG(纯化)
Cat:SPA131
Solarbio
宿主:Goat
交叉反应:Mouse
应用:ELISA WB
稀释比例:WB 1:1000-20000.;ELISA:1:5000-100000.
储存条件:Shipped at 4℃, Store at -2
英文名称
Goat Anti-Mouse IgG(Purified Antibody)
中文名称
羊抗小鼠IgG(纯化抗体)
规格
10mg ; 100mg ; 1mg
宿主
Goat
克隆类型
Polyclonal Antibody
亚型
IgG
交叉反应
Mouse
应用
ELISA WB
稀释比例
WB 1:1000-20000.;ELISA:1:5000-100000.
抗体信息
背景说明
Immunoglobulin G (IgG),is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
免疫原
Mouse IgG full length protein
理论分子量
150kDa
其它标识
纯化方法
Affinity purification
储存液
Buffer: PBS with 0.03% Proclin300(PH7.2-7.4).
储存条件
Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).
外观(性状)
Liquid
浓度
10mg/mL
产品介绍
Product Introduction
采用硫酸铵盐析与DEAE-离子交换纤维素层析,或批次吸附纯化,制成高纯度(﹥95%)和高活性的纯化IgG抗体。各种纯化抗体均为液体提供,可根据用户要求,配成不同浓度,用前最好-20℃存放,可根据使用方法采用不同浓度稀释。通常不同使用目的可分别用下列缓冲液稀释,注意完全化冻,充分混匀。用前最好低温高速离心去除冷聚合球蛋白及变性蛋白后,重新定量。
金标记:用pH9.0硼酸缓冲液稀释,1~2mg/ml标记。
HRP标记:用0.01mol/L pH9.0、CB或用包被液CB稀释,6~8mg/ml标记
荧光标记:用0.5mol/L pH9.5、CB(碳酸盐缓冲液)稀释,10~20 mg/ml标记。
生物素记抗体:用0.1mol/L NaHCO3稀释,1~2mg/ml标记。其它方法,如免疫扩散可用0.01mol/L pH7.2 PBS稀释,ELISA包被10~20μg/ml 4℃过夜或37℃ 2h。
备注:产品信息可能会有优化升级。请以实际标签信息为准。